Slow freezing and vitrification of mouse morula and early blastocysts
نویسندگان
چکیده
منابع مشابه
Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage
Cryopreservation is known for its marked deleterious effects on embryonic health. Bovine compact morulae were vitrified or slow-frozen, and post-warm morulae were cultured to the expanded blastocyst stage. Blastocysts developed from vitrified and slow-frozen morulae were subjected to microarray analysis and compared with blastocysts developed from unfrozen control morulae for differential gene ...
متن کاملDifferent chromatin and energy/redox responses of mouse morulae and blastocysts to slow freezing and vitrification
BACKGROUND The ability to cryopreserve mammalian embryos has become an integral part of assisted reproduction, both in human and veterinary medicine. Despite differences in the size and physiological characteristics of embryos from various species, the embryos have been frozen by either of two procedures: slow freezing or vitrification. The aim of our study was to compare the effect of slow fre...
متن کاملClinical outcomes following cryopreservation of blastocysts by vitrification or slow freezing: a population-based cohort study.
STUDY QUESTION What are the clinical efficacy and perinatal outcomes following transfer of vitrified blastocysts compared with transfer of fresh or of slow frozen blastocysts? SUMMARY ANSWER Compared with slow frozen blastocysts, vitrified blastocysts resulted in significantly higher clinical pregnancy and live delivery rates with similar perinatal outcomes at population level. WHAT IS KNOW...
متن کاملdevelopmental potetial of mouse morula early and late blastocyst after vitrification
introduction: the objective of this study was to assess developmental potential of mouse morulae, early and laic blastocysts after vitrification in r2 and r2+vero mediums. material and methods: morulae, early and late blastocysts were obtained from superovulated nmri female mice. embryos in experimental group were suspended in a solution of ethylene glycol, ficoll and sucrose (efs40) for 2 minu...
متن کاملEvaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing.
OBJECTIVE To evaluate the effect of vitrification and two other methods of slow cryopreservation on DNA integrity in expanded and nonexpanded blastocysts. DESIGN Prospective in vitro study. SETTING Tertiary care academic hospital. INTERVENTION(S) 1) Twenty-two expanded blastocysts (EB) and 17 nonexpanded blastocysts (NEB) vitrified in cryotips; 2) 15 EB and 16 NEB by slow freezing using p...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2013
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-013-0056-4